3rr5

X-ray diffraction
3.02Å resolution

DNA ligase from the archaeon Thermococcus sp. 1519

Released:

Function and Biology Details

Reaction catalysed:
(1a) ATP + [DNA ligase]-L-lysine = 5'-adenosyl [DNA ligase]-N(epsilon)-phosphono-L-lysine + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA ligase Chain: A
Molecule details ›
Chain: A
Length: 570 amino acids
Theoretical weight: 64.89 KDa
Source organism: Thermococcus sp. 1519
Expression system: Escherichia coli
UniProt:
  • Canonical: C0LJI8 (Residues: 2-559; Coverage: 100%)
Gene name: lig
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 76.95Å b: 85.6Å c: 105.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.233 0.31
Expression system: Escherichia coli