› 3rpq

X-ray diffraction
2.61Å resolution

Domain-domain flexibility leads to allostery within the camp receptor protein (CRP)

Released:
DOI: 10.2210/pdb3rpq/pdb
PDB 3rpq coloured by chain and viewed from the front.
PDB 3rpq coloured by chain and viewed from the side.
PDB 3rpq coloured by chain and viewed from the top.
Source organism: Escherichia coli K-12
Entry authors: Knapp J, White MA, Lee JC

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-activated global transcriptional regulator CRP Chains: A, B
Molecule details ›
Chains: A, B
Length: 210 amino acids
Theoretical weight: 23.73 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACJ8 (Residues: 1-210; Coverage: 100%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CMP 4 x CMP
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: P21
Unit cell:
a: 45.59Å b: 102.81Å c: 54.24Å
α: 90° β: 110.81° γ: 90°
R-values:
R R work R free
0.213 0.206 0.258
Expression system: Escherichia coli

Quick links