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Structures of native and Fe-substituted SOD2 from Saccharomyces cerevisiae.

Kang Y, He YX, Zhao MX, Li WF
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 1173-8 (2011)
Cited: 10 times
EuropePMC logo PMID: 22102021

Abstract

The manganese-specific superoxide dismutase SOD2 from the yeast Saccharomyces cerevisiae is a protein that resides in the mitochondrion and protects it against attack by superoxide radicals. However, a high iron concentration in the mitochondria results in iron misincorporation at the active site, with subsequent inactivation of SOD2. Here, the crystal structures of SOD2 bound with the native metal manganese and with the `wrong' metal iron are presented at 2.05 and 1.79 Å resolution, respectively. Structural comparison of the two structures shows no significant conformational alteration in the overall structure or in the active site upon binding the non-native metal iron. Moreover, residues Asp163 and Lys80 are proposed to potentially be responsible for the metal specificity of the Mn-specific SOD. Additionally, the surface-potential distribution of SOD2 revealed a conserved positively charged electrostatic zone in the proximity of the active site that probably functions in the same way as in Cu/Zn-SODs by facilitating the diffusion of the superoxide anion to the metal ion.

Articles - 3rn4 mentioned but not cited (1)

  1. Structures of native and Fe-substituted SOD2 from Saccharomyces cerevisiae. Kang Y, He YX, Zhao MX, Li WF. Acta Crystallogr Sect F Struct Biol Cryst Commun 67 1173-1178 (2011)


Reviews citing this publication (4)

  1. Battles with iron: manganese in oxidative stress protection. Aguirre JD, Culotta VC. J Biol Chem 287 13541-13548 (2012)
  2. Insights into the Dichotomous Regulation of SOD2 in Cancer. Kim YS, Gupta Vallur P, Phaëton R, Mythreye K, Hempel N. Antioxidants (Basel) 6 E86 (2017)
  3. Influence of iron metabolism on manganese transport and toxicity. Ye Q, Park JE, Gugnani K, Betharia S, Pino-Figueroa A, Kim J. Metallomics 9 1028-1046 (2017)
  4. Interchangeable utilization of metals: New perspectives on the impacts of metal ions employed in ancient and extant biomolecules. Smethurst DGJ, Shcherbik N. J Biol Chem 297 101374 (2021)

Articles citing this publication (5)

  1. A manganese-rich environment supports superoxide dismutase activity in a Lyme disease pathogen, Borrelia burgdorferi. Aguirre JD, Clark HM, McIlvin M, Vazquez C, Palmere SL, Grab DJ, Seshu J, Hart PJ, Saito M, Culotta VC. J Biol Chem 288 8468-8478 (2013)
  2. Lysine 68 acetylation directs MnSOD as a tetrameric detoxification complex versus a monomeric tumor promoter. Zhu Y, Zou X, Dean AE, Brien JO, Gao Y, Tran EL, Park SH, Liu G, Kieffer MB, Jiang H, Stauffer ME, Hart R, Quan S, Satchell KJF, Horikoshi N, Bonini M, Gius D. Nat Commun 10 2399 (2019)
  3. Insights into the iron-ome and manganese-ome of Δmtm1 Saccharomyces cerevisiae mitochondria. Park J, McCormick SP, Chakrabarti M, Lindahl PA. Metallomics 5 656-672 (2013)
  4. MTM1 displays a new function in the regulation of nickel resistance in Saccharomyces cerevisiae. Xu N, Xu Y, Smith N, Chen H, Guo Z, Lee J, Wu X. Metallomics 14 mfac074 (2022)
  5. Disruption in iron homeostasis and impaired activity of iron-sulfur cluster containing proteins in the yeast model of Shwachman-Diamond syndrome. Jain A, Nilatawong P, Mamak N, Jensen LT, Jensen AN. Cell Biosci 10 105 (2020)


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