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Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to MgAMPPNP

Source organism: Escherichia coli K-12
Biochemical function: protein binding
Biological process: organic substance transport
Cellular component: maltose transport complex

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EC 7.5.2.1: ABC-type maltose transporter

Reaction catalysed:
ATP + H(2)O + maltose-[maltose-binding protein](Side 1) = ADP + phosphate + maltose(Side 2) + [maltose-binding protein](Side 1)
Systematic name:
ATP phosphohydrolase (ABC-type, maltose-importing)
Alternative Name(s):
  • Maltose ABC transporter
  • Maltose-transporting ATPase

GO terms

Sequence families

Pfam Protein families (Pfam)
PF00528
Domain description: Binding-protein-dependent transport system inner membrane component
Occurring in:
  1. Maltose/maltodextrin transport system permease protein MalF
  2. Maltose/maltodextrin transport system permease protein MalG
The deposited structure of PDB entry 3rlf contains 1 copy of Pfam domain PF00528 (Binding-protein-dependent transport system inner membrane component) in Maltose/maltodextrin transport system permease protein MalF. Showing 1 copy in chain B [auth F].
The deposited structure of PDB entry 3rlf contains 1 copy of Pfam domain PF00528 (Binding-protein-dependent transport system inner membrane component) in Maltose/maltodextrin transport system permease protein MalG. Showing 1 copy in chain C [auth G].

PF08402
Domain description: TOBE domain
Occurring in:
  1. Maltose/maltodextrin import ATP-binding protein MalK

PF00005
Domain description: ABC transporter
Occurring in:
  1. Maltose/maltodextrin import ATP-binding protein MalK
The deposited structure of PDB entry 3rlf contains 2 copies of Pfam domain PF00005 (ABC transporter) in Maltose/maltodextrin import ATP-binding protein MalK. Showing 1 copy in chain D [auth A].

PF14785
Domain description: Maltose transport system permease protein MalF P2 domain
Occurring in:
  1. Maltose/maltodextrin transport system permease protein MalF
The deposited structure of PDB entry 3rlf contains 1 copy of Pfam domain PF14785 (Maltose transport system permease protein MalF P2 domain) in Maltose/maltodextrin transport system permease protein MalF. Showing 1 copy in chain B [auth F].

PF20872
Domain description: MalF, N-terminal region, transmembrane helices
Occurring in:
  1. Maltose/maltodextrin transport system permease protein MalF

PF01547
Domain description: Bacterial extracellular solute-binding protein
Occurring in:
  1. Maltose/maltodextrin-binding periplasmic protein
The deposited structure of PDB entry 3rlf contains 1 copy of Pfam domain PF01547 (Bacterial extracellular solute-binding protein) in Maltose/maltodextrin-binding periplasmic protein. Showing 1 copy in chain A [auth E].

InterPro InterPro annotations
IPR006060
Domain description: Maltose/Cyclodextrin ABC transporter, substrate-binding protein
Occurring in:
  1. Maltose/maltodextrin-binding periplasmic protein
IPR000515
Domain description: ABC transporter type 1, transmembrane domain MetI-like
Occurring in:
  1. Maltose/maltodextrin transport system permease protein MalF
  2. Maltose/maltodextrin transport system permease protein MalG
IPR035906
Domain description: MetI-like superfamily
Occurring in:
  1. Maltose/maltodextrin transport system permease protein MalF
  2. Maltose/maltodextrin transport system permease protein MalG
IPR003439
Domain description: ABC transporter-like, ATP-binding domain
Occurring in:
  1. Maltose/maltodextrin import ATP-binding protein MalK
IPR027417
Domain description: P-loop containing nucleoside triphosphate hydrolase
Occurring in:
  1. Maltose/maltodextrin import ATP-binding protein MalK
IPR012340
Domain description: Nucleic acid-binding, OB-fold
Occurring in:
  1. Maltose/maltodextrin import ATP-binding protein MalK
IPR008995
Domain description: Molybdate/tungstate binding, C-terminal
Occurring in:
  1. Maltose/maltodextrin import ATP-binding protein MalK
IPR040582
Domain description: MalK-like, OB fold domain
Occurring in:
  1. Maltose/maltodextrin import ATP-binding protein MalK
IPR017871
Domain description: ABC transporter-like, conserved site
Occurring in:
  1. Maltose/maltodextrin import ATP-binding protein MalK
IPR003593
Domain description: AAA+ ATPase domain
Occurring in:
  1. Maltose/maltodextrin import ATP-binding protein MalK
IPR015855
Domain description: ABC transporter, maltose/maltodextrin import, MalK-like
Occurring in:
  1. Maltose/maltodextrin import ATP-binding protein MalK
IPR047641
Domain description: ABC transporter, ATP-binding protein MalK/UgpC-like
Occurring in:
  1. Maltose/maltodextrin import ATP-binding protein MalK
IPR047103
Domain description: Maltose transport system permease protein MalF, P2 domain superfamily
Occurring in:
  1. Maltose/maltodextrin transport system permease protein MalF
IPR029345
Domain description: Maltose transport system permease protein MalF, P2 domain
Occurring in:
  1. Maltose/maltodextrin transport system permease protein MalF
IPR035277
Domain description: MalF, N-terminal
Occurring in:
  1. Maltose/maltodextrin transport system permease protein MalF
IPR006061
Domain description: Solute-binding family 1, conserved site
Occurring in:
  1. Maltose/maltodextrin-binding periplasmic protein
IPR006059
Domain description: Bacterial-type extracellular solute-binding protein
Occurring in:
  1. Maltose/maltodextrin-binding periplasmic protein

Structure domains

CATH CATH domains
3.40.190.10
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: D-Maltodextrin-Binding Protein; domain 2
Homology: Periplasmic binding protein-like II
Occurring in:
  1. Maltose/maltodextrin-binding periplasmic protein
The deposited structure of PDB entry 3rlf contains 2 copies of CATH domain 3.40.190.10 (D-Maltodextrin-Binding Protein; domain 2) in Maltose/maltodextrin-binding periplasmic protein. Showing 2 copies in chain A [auth E].
2.40.50.100
Class: Mainly Beta
Architecture: Beta Barrel
Topology: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Homology: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Occurring in:
  1. Maltose/maltodextrin import ATP-binding protein MalK
The deposited structure of PDB entry 3rlf contains 2 copies of CATH domain 2.40.50.100 (OB fold (Dihydrolipoamide Acetyltransferase, E2P)) in Maltose/maltodextrin import ATP-binding protein MalK. Showing 1 copy in chain D [auth A].
1.10.3720.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: MetI-like fold
Homology: MetI-like
Occurring in:
  1. Maltose/maltodextrin transport system permease protein MalF
  2. Maltose/maltodextrin transport system permease protein MalG
The deposited structure of PDB entry 3rlf contains 1 copy of CATH domain 1.10.3720.10 (MetI-like fold) in Maltose/maltodextrin transport system permease protein MalF. Showing 1 copy in chain B [auth F].
The deposited structure of PDB entry 3rlf contains 1 copy of CATH domain 1.10.3720.10 (MetI-like fold) in Maltose/maltodextrin transport system permease protein MalG. Showing 1 copy in chain C [auth G].
3.10.650.10
Class: Alpha Beta
Architecture: Roll
Topology: MalF N-terminal region-like
Homology: MalF N-terminal region-like
Occurring in:
  1. Maltose/maltodextrin transport system permease protein MalF
The deposited structure of PDB entry 3rlf contains 1 copy of CATH domain 3.10.650.10 (MalF N-terminal region-like) in Maltose/maltodextrin transport system permease protein MalF. Showing 1 copy in chain B [auth F].
2.40.50.140
Class: Mainly Beta
Architecture: Beta Barrel
Topology: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Homology: Nucleic acid-binding proteins
Occurring in:
  1. Maltose/maltodextrin import ATP-binding protein MalK
The deposited structure of PDB entry 3rlf contains 2 copies of CATH domain 2.40.50.140 (OB fold (Dihydrolipoamide Acetyltransferase, E2P)) in Maltose/maltodextrin import ATP-binding protein MalK. Showing 1 copy in chain D [auth A].
3.40.50.300
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: P-loop containing nucleotide triphosphate hydrolases
Occurring in:
  1. Maltose/maltodextrin import ATP-binding protein MalK
The deposited structure of PDB entry 3rlf contains 2 copies of CATH domain 3.40.50.300 (Rossmann fold) in Maltose/maltodextrin import ATP-binding protein MalK. Showing 1 copy in chain D [auth A].
2.40.430.10
Class: Mainly Beta
Architecture: Beta Barrel
Topology: Periplasmic binding protein-like II
Homology: D-maltodextrin-binding protein, MBP
Occurring in:
  1. Maltose/maltodextrin transport system permease protein MalF
The deposited structure of PDB entry 3rlf contains 1 copy of CATH domain 2.40.430.10 (Periplasmic binding protein-like II) in Maltose/maltodextrin transport system permease protein MalF. Showing 1 copy in chain B [auth F].
1.20.58.370
Class: Mainly Alpha
Architecture: Up-down Bundle
Topology: Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homology: MalF N-terminal region-like
Occurring in:
  1. Maltose/maltodextrin transport system permease protein MalF
The deposited structure of PDB entry 3rlf contains 1 copy of CATH domain 1.20.58.370 (Methane Monooxygenase Hydroxylase; Chain G, domain 1) in Maltose/maltodextrin transport system permease protein MalF. Showing 1 copy in chain B [auth F].

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