› 3rku

X-ray diffraction
2.6Å resolution

Substrate Fingerprint and the Structure of NADP+ Dependent Serine Dehydrogenase from Saccharomyces cerevisiae complexed with NADP+

Released:
DOI: 10.2210/pdb3rku/pdb
PDB entry 3rku coloured by chain, front view.
PDB entry 3rku coloured by chain, side view.
PDB entry 3rku coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Entry authors: Huether R, Pacheco CM, Duax WL

Function and Biology Details

Reaction catalysed: 
(1a) L-allo-threonine + NADP(+) = L-2-amino-3-oxobutanoate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170120 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent 3-hydroxy acid dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 287 amino acids
Theoretical weight: 31.45 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05016 (Residues: 1-267; Coverage: 100%)
Gene names: YM9959.08C, YMR226C
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 82.39Å b: 87.25Å c: 83.81Å
α: 90° β: 112.98° γ: 90°
R-values:
R R work R free
0.191 0.188 0.245
Expression system: Escherichia coli

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