3rkr

X-ray diffraction
2.42Å resolution

Function and Biology Details

Reaction catalysed:
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Short chain oxidoreductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 262 amino acids
Theoretical weight: 27.43 KDa
Source organism: uncultured bacterium Bio5
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B2BKB1 (Residues: 1-239; Coverage: 100%)
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: I422
Unit cell:
a: 243.167Å b: 243.167Å c: 151.549Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.222
Expression system: Escherichia coli BL21