3rk3 › Citations

Complexin cross-links prefusion SNAREs into a zigzag array.

Kümmel D, Krishnakumar SS, Radoff DT, Li F, Giraudo CG, Pincet F, Rothman JE, Reinisch KM
Nat Struct Mol Biol 18 927-33 (2011)
Related entries: 3rk2, 3rl0

Cited: 116 times
EuropePMC logo PMID: 21785414

Abstract

Complexin prevents SNAREs from releasing neurotransmitters until an action potential arrives at the synapse. To understand the mechanism for this inhibition, we determined the structure of complexin bound to a mimetic of a prefusion SNAREpin lacking the portion of the v-SNARE that zippers last to trigger fusion. The 'central helix' of complexin is anchored to one SNARE complex, while its 'accessory helix' extends away at ~45° and bridges to a second complex, occupying the vacant v-SNARE binding site to inhibit fusion. We expected the accessory helix to compete with the v-SNARE for t-SNARE binding but found instead that the interaction occurs intermolecularly. Thus, complexin organizes the SNAREs into a zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion.

Reviews - 3rk3 mentioned but not cited (2)

  1. Mechanism of neurotransmitter release coming into focus. Rizo J. Protein Sci 27 1364-1391 (2018)
  2. Molecular Mechanisms of Fast Neurotransmitter Release. Brunger AT, Choi UB, Lai Y, Leitz J, Zhou Q. Annu Rev Biophys 47 469-497 (2018)

Articles - 3rk3 mentioned but not cited (8)

  1. The primed SNARE-complexin-synaptotagmin complex for neuronal exocytosis. Zhou Q, Zhou P, Wang AL, Wu D, Zhao M, Südhof TC, Brunger AT. Nature 548 420-425 (2017)
  2. Complexin cross-links prefusion SNAREs into a zigzag array. Kümmel D, Krishnakumar SS, Radoff DT, Li F, Giraudo CG, Pincet F, Rothman JE, Reinisch KM. Nat Struct Mol Biol 18 927-933 (2011)
  3. Re-examining how complexin inhibits neurotransmitter release. Trimbuch T, Xu J, Flaherty D, Tomchick DR, Rizo J, Rosenmund C. Elife 3 e02391 (2014)
  4. Complexin induces a conformational change at the membrane-proximal C-terminal end of the SNARE complex. Choi UB, Zhao M, Zhang Y, Lai Y, Brunger AT. Elife 5 e16886 (2016)
  5. Stabilization of spontaneous neurotransmitter release at ribbon synapses by ribbon-specific subtypes of complexin. Vaithianathan T, Zanazzi G, Henry D, Akmentin W, Matthews G. J Neurosci 33 8216-8226 (2013)
  6. Reconciling isothermal titration calorimetry analyses of interactions between complexin and truncated SNARE complexes. Prinslow EA, Brautigam CA, Rizo J. Elife 6 e30286 (2017)
  7. Arrangements of proteins at reconstituted synaptic vesicle fusion sites depend on membrane separation. Ginger L, Malsam J, Sonnen AF, Morado D, Scheutzow A, Söllner TH, Briggs JAG. FEBS Lett 594 3450-3463 (2020)
  8. Hypothesis Relating the Structure, Biochemistry and Function of Active Zone Material Macromolecules at a Neuromuscular Junction. Szule JA. Front Synaptic Neurosci 13 798225 (2021)


Reviews citing this publication (28)

  1. Neurotransmitter release: the last millisecond in the life of a synaptic vesicle. Südhof TC. Neuron 80 675-690 (2013)
  2. Molecular machines governing exocytosis of synaptic vesicles. Jahn R, Fasshauer D. Nature 490 201-207 (2012)
  3. The Synaptic Vesicle Release Machinery. Rizo J, Xu J. Annu Rev Biophys 44 339-367 (2015)
  4. Chaperoning SNARE assembly and disassembly. Baker RW, Hughson FM. Nat Rev Mol Cell Biol 17 465-479 (2016)
  5. The Multifaceted Role of SNARE Proteins in Membrane Fusion. Han J, Pluhackova K, Böckmann RA. Front Physiol 8 5 (2017)
  6. Distinct initial SNARE configurations underlying the diversity of exocytosis. Kasai H, Takahashi N, Tokumaru H. Physiol Rev 92 1915-1964 (2012)
  7. Should I stop or should I go? The role of complexin in neurotransmitter release. Trimbuch T, Rosenmund C. Nat Rev Neurosci 17 118-125 (2016)
  8. GPCR mediated regulation of synaptic transmission. Betke KM, Wells CA, Hamm HE. Prog Neurobiol 96 304-321 (2012)
  9. The blockade of the neurotransmitter release apparatus by botulinum neurotoxins. Pantano S, Montecucco C. Cell Mol Life Sci 71 793-811 (2014)
  10. Hypothesis - buttressed rings assemble, clamp, and release SNAREpins for synaptic transmission. Rothman JE, Krishnakumar SS, Grushin K, Pincet F. FEBS Lett 591 3459-3480 (2017)
  11. How could SNARE proteins open a fusion pore? Fang Q, Lindau M. Physiology (Bethesda) 29 278-285 (2014)
  12. Complexins: small but capable. Mohrmann R, Dhara M, Bruns D. Cell Mol Life Sci 72 4221-4235 (2015)
  13. Three steps forward, two steps back: mechanistic insights into the assembly and disassembly of the SNARE complex. Bombardier JP, Munson M. Curr Opin Chem Biol 29 66-71 (2015)
  14. Ca2+-Triggered Synaptic Vesicle Fusion Initiated by Release of Inhibition. Brunger AT, Leitz J, Zhou Q, Choi UB, Lai Y. Trends Cell Biol 28 631-645 (2018)
  15. The impact of bacterial infection on mast cell degranulation. Wesolowski J, Paumet F. Immunol Res 51 215-226 (2011)
  16. Molecular Mechanisms Underlying Neurotransmitter Release. Rizo J. Annu Rev Biophys 51 377-408 (2022)
  17. SNARE requirements en route to exocytosis: from many to few. Mohrmann R, Sørensen JB. J Mol Neurosci 48 387-394 (2012)
  18. SNARE zippering. Lou X, Shin YK. Biosci Rep 36 e00327 (2016)
  19. Enlightening molecular mechanisms through study of protein interactions. Rizo J, Rosen MK, Gardner KH. J Mol Cell Biol 4 270-283 (2012)
  20. SNARE Regulatory Proteins in Synaptic Vesicle Fusion and Recycling. Sauvola CW, Littleton JT. Front Mol Neurosci 14 733138 (2021)
  21. The proteins of exocytosis: lessons from the sperm model. Tomes CN. Biochem J 465 359-370 (2015)
  22. Vesicular Release of GABA by Mammalian Horizontal Cells Mediates Inhibitory Output to Photoreceptors. Hirano AA, Vuong HE, Kornmann HL, Schietroma C, Stella SL, Barnes S, Brecha NC. Front Cell Neurosci 14 600777 (2020)
  23. v-SNARE function in chromaffin cells. Dhara M, Mohrmann R, Bruns D. Pflugers Arch 470 169-180 (2018)
  24. Vesicle trafficking and vesicle fusion: mechanisms, biological functions, and their implications for potential disease therapy. Cui L, Li H, Xi Y, Hu Q, Liu H, Fan J, Xiang Y, Zhang X, Shui W, Lai Y. Mol Biomed 3 29 (2022)
  25. On the difficulties of characterizing weak protein interactions that are critical for neurotransmitter release. Rizo J, David G, Fealey ME, Jaczynska K. FEBS Open Bio 12 1912-1938 (2022)
  26. Complexin Membrane Interactions: Implications for Synapse Evolution and Function. Lottermoser JA, Dittman JS. J Mol Biol 435 167774 (2023)
  27. Mechanisms of SNARE proteins in membrane fusion. Jahn R, Cafiso DC, Tamm LK. Nat Rev Mol Cell Biol (2023)
  28. Molecular Dynamics Simulations of the Proteins Regulating Synaptic Vesicle Fusion. Bykhovskaia M. Membranes (Basel) 13 307 (2023)

Articles citing this publication (78)

  1. Single reconstituted neuronal SNARE complexes zipper in three distinct stages. Gao Y, Zorman S, Gundersen G, Xi Z, Ma L, Sirinakis G, Rothman JE, Zhang Y. Science 337 1340-1343 (2012)
  2. Synaptic proteins promote calcium-triggered fast transition from point contact to full fusion. Diao J, Grob P, Cipriano DJ, Kyoung M, Zhang Y, Shah S, Nguyen A, Padolina M, Srivastava A, Vrljic M, Shah A, Nogales E, Chu S, Brunger AT. Elife 1 e00109 (2012)
  3. Complexin controls spontaneous and evoked neurotransmitter release by regulating the timing and properties of synaptotagmin activity. Jorquera RA, Huntwork-Rodriguez S, Akbergenova Y, Cho RW, Littleton JT. J Neurosci 32 18234-18245 (2012)
  4. An extended helical conformation in domain 3a of Munc18-1 provides a template for SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex assembly. Parisotto D, Pfau M, Scheutzow A, Wild K, Mayer MP, Malsam J, Sinning I, Söllner TH. J Biol Chem 289 9639-9650 (2014)
  5. Complexin arrests a pool of docked vesicles for fast Ca2+-dependent release. Malsam J, Parisotto D, Bharat TA, Scheutzow A, Krause JM, Briggs JA, Söllner TH. EMBO J 31 3270-3281 (2012)
  6. The UPR Activator ATF6 Responds to Proteotoxic and Lipotoxic Stress by Distinct Mechanisms. Tam AB, Roberts LS, Chandra V, Rivera IG, Nomura DK, Forbes DJ, Niwa M. Dev Cell 46 327-343.e7 (2018)
  7. Complexin inhibits spontaneous release and synchronizes Ca2+-triggered synaptic vesicle fusion by distinct mechanisms. Lai Y, Diao J, Cipriano DJ, Zhang Y, Pfuetzner RA, Padolina MS, Brunger AT. Elife 3 e03756 (2014)
  8. The principle of membrane fusion in the cell (Nobel lecture). Rothman JE. Angew Chem Int Ed Engl 53 12676-12694 (2014)
  9. Synaptic vesicles position complexin to block spontaneous fusion. Wragg RT, Snead D, Dong Y, Ramlall TF, Menon I, Bai J, Eliezer D, Dittman JS. Neuron 77 323-334 (2013)
  10. Common intermediates and kinetics, but different energetics, in the assembly of SNARE proteins. Zorman S, Rebane AA, Ma L, Yang G, Molski MA, Coleman J, Pincet F, Rothman JE, Zhang Y. Elife 3 e03348 (2014)
  11. Calcium sensitive ring-like oligomers formed by synaptotagmin. Wang J, Bello O, Auclair SM, Wang J, Coleman J, Pincet F, Krishnakumar SS, Sindelar CV, Rothman JE. Proc Natl Acad Sci U S A 111 13966-13971 (2014)
  12. Comparative studies of Munc18c and Munc18-1 reveal conserved and divergent mechanisms of Sec1/Munc18 proteins. Yu H, Rathore SS, Lopez JA, Davis EM, James DE, Martin JL, Shen J. Proc Natl Acad Sci U S A 110 E3271-80 (2013)
  13. Membrane curvature sensing by the C-terminal domain of complexin. Snead D, Wragg RT, Dittman JS, Eliezer D. Nat Commun 5 4955 (2014)
  14. Complexin synchronizes primed vesicle exocytosis and regulates fusion pore dynamics. Dhara M, Yarzagaray A, Schwarz Y, Dutta S, Grabner C, Moghadam PK, Bost A, Schirra C, Rettig J, Reim K, Brose N, Mohrmann R, Bruns D. J Cell Biol 204 1123-1140 (2014)
  15. Munc18 and Munc13 serve as a functional template to orchestrate neuronal SNARE complex assembly. Wang S, Li Y, Gong J, Ye S, Yang X, Zhang R, Ma C. Nat Commun 10 69 (2019)
  16. A half-zippered SNARE complex represents a functional intermediate in membrane fusion. Li F, Kümmel D, Coleman J, Reinisch KM, Rothman JE, Pincet F. J Am Chem Soc 136 3456-3464 (2014)
  17. Genetic analysis of the Complexin trans-clamping model for cross-linking SNARE complexes in vivo. Cho RW, Kümmel D, Li F, Baguley SW, Coleman J, Rothman JE, Littleton JT. Proc Natl Acad Sci U S A 111 10317-10322 (2014)
  18. C-terminal domain of mammalian complexin-1 localizes to highly curved membranes. Gong J, Lai Y, Li X, Wang M, Leitz J, Hu Y, Zhang Y, Choi UB, Cipriano D, Pfuetzner RA, Südhof TC, Yang X, Brunger AT, Diao J. Proc Natl Acad Sci U S A 113 E7590-E7599 (2016)
  19. The trans-SNARE-regulating function of Munc18-1 is essential to synaptic exocytosis. Shen C, Rathore SS, Yu H, Gulbranson DR, Hua R, Zhang C, Schoppa NE, Shen J. Nat Commun 6 8852 (2015)
  20. Impaired neurotransmission in ether lipid-deficient nerve terminals. Brodde A, Teigler A, Brugger B, Lehmann WD, Wieland F, Berger J, Just WW. Hum Mol Genet 21 2713-2724 (2012)
  21. Ring-like oligomers of Synaptotagmins and related C2 domain proteins. Zanetti MN, Bello OD, Wang J, Coleman J, Cai Y, Sindelar CV, Rothman JE, Krishnakumar SS. Elife 5 e17262 (2016)
  22. SNARE and regulatory proteins induce local membrane protrusions to prime docked vesicles for fast calcium-triggered fusion. Bharat TA, Malsam J, Hagen WJ, Scheutzow A, Söllner TH, Briggs JA. EMBO Rep 15 308-314 (2014)
  23. Complexin-1 enhances the on-rate of vesicle docking via simultaneous SNARE and membrane interactions. Diao J, Cipriano DJ, Zhao M, Zhang Y, Shah S, Padolina MS, Pfuetzner RA, Brunger AT. J Am Chem Soc 135 15274-15277 (2013)
  24. Interaction of the complexin accessory helix with the C-terminus of the SNARE complex: molecular-dynamics model of the fusion clamp. Bykhovskaia M, Jagota A, Gonzalez A, Vasin A, Littleton JT. Biophys J 105 679-690 (2013)
  25. Conformational dynamics of calcium-triggered activation of fusion by synaptotagmin. Krishnakumar SS, Kümmel D, Jones SJ, Radoff DT, Reinisch KM, Rothman JE. Biophys J 105 2507-2516 (2013)
  26. Morphologies of synaptic protein membrane fusion interfaces. Gipson P, Fukuda Y, Danev R, Lai Y, Chen DH, Baumeister W, Brunger AT. Proc Natl Acad Sci U S A 114 9110-9115 (2017)
  27. N-terminal domain of complexin independently activates calcium-triggered fusion. Lai Y, Choi UB, Zhang Y, Zhao M, Pfuetzner RA, Wang AL, Diao J, Brunger AT. Proc Natl Acad Sci U S A 113 E4698-707 (2016)
  28. Dynamic flux of microvesicles modulate parasite-host cell interaction of Trypanosoma cruzi in eukaryotic cells. Ramirez MI, Deolindo P, de Messias-Reason IJ, Arigi EA, Choi H, Almeida IC, Evans-Osses I. Cell Microbiol 19 (2017)
  29. Involvement of complexin 2 in docking, locking and unlocking of different SNARE complexes during sperm capacitation and induced acrosomal exocytosis. Tsai PS, Brewis IA, van Maaren J, Gadella BM. PLoS One 7 e32603 (2012)
  30. Kinetic barriers to SNAREpin assembly in the regulation of membrane docking/priming and fusion. Li F, Tiwari N, Rothman JE, Pincet F. Proc Natl Acad Sci U S A 113 10536-10541 (2016)
  31. SNAREpin assembly by Munc18-1 requires previous vesicle docking by synaptotagmin 1. Parisotto D, Malsam J, Scheutzow A, Krause JM, Söllner TH. J Biol Chem 287 31041-31049 (2012)
  32. The accessory helix of complexin functions by stabilizing central helix secondary structure. Radoff DT, Dong Y, Snead D, Bai J, Eliezer D, Dittman JS. Elife 3 (2014)
  33. Stability, folding dynamics, and long-range conformational transition of the synaptic t-SNARE complex. Zhang X, Rebane AA, Ma L, Li F, Jiao J, Qu H, Pincet F, Rothman JE, Zhang Y. Proc Natl Acad Sci U S A 113 E8031-E8040 (2016)
  34. Two coiled-coil domains of Chlamydia trachomatis IncA affect membrane fusion events during infection. Ronzone E, Paumet F. PLoS One 8 e69769 (2013)
  35. Sec17/Sec18 act twice, enhancing membrane fusion and then disassembling cis-SNARE complexes. Song H, Orr A, Duan M, Merz AJ, Wickner W. Elife 6 e26646 (2017)
  36. Structural disorder provides increased adaptability for vesicle trafficking pathways. Pietrosemoli N, Pancsa R, Tompa P. PLoS Comput Biol 9 e1003144 (2013)
  37. Structural basis for the clamping and Ca2+ activation of SNARE-mediated fusion by synaptotagmin. Grushin K, Wang J, Coleman J, Rothman JE, Sindelar CV, Krishnakumar SS. Nat Commun 10 2413 (2019)
  38. Dual roles of Munc18-1 rely on distinct binding modes of the central cavity with Stx1A and SNARE complex. Shi L, Kümmel D, Coleman J, Melia TJ, Giraudo CG. Mol Biol Cell 22 4150-4160 (2011)
  39. Reconstituting Intracellular Vesicle Fusion Reactions: The Essential Role of Macromolecular Crowding. Yu H, Rathore SS, Shen C, Liu Y, Ouyang Y, Stowell MH, Shen J. J Am Chem Soc 137 12873-12883 (2015)
  40. Phosphatidylinositol 4,5-biphosphate (PIP(2)) lipids regulate the phosphorylation of syntaxin N-terminus by modulating both its position and local structure. Khelashvili G, Galli A, Weinstein H. Biochemistry 51 7685-7698 (2012)
  41. Unique Structural Features of Membrane-Bound C-Terminal Domain Motifs Modulate Complexin Inhibitory Function. Snead D, Lai AL, Wragg RT, Parisotto DA, Ramlall TF, Dittman JS, Freed JH, Eliezer D. Front Mol Neurosci 10 154 (2017)
  42. Complexin 3 Increases the Fidelity of Signaling in a Retinal Circuit by Regulating Exocytosis at Ribbon Synapses. Mortensen LS, Park SJH, Ke JB, Cooper BH, Zhang L, Imig C, Löwel S, Reim K, Brose N, Demb JB, Rhee JS, Singer JH. Cell Rep 15 2239-2250 (2016)
  43. Complexin Binding to Membranes and Acceptor t-SNAREs Explains Its Clamping Effect on Fusion. Zdanowicz R, Kreutzberger A, Liang B, Kiessling V, Tamm LK, Cafiso DS. Biophys J 113 1235-1250 (2017)
  44. Gβγ inhibits exocytosis via interaction with critical residues on soluble N-ethylmaleimide-sensitive factor attachment protein-25. Wells CA, Zurawski Z, Betke KM, Yim YY, Hyde K, Rodriguez S, Alford S, Hamm HE. Mol Pharmacol 82 1136-1149 (2012)
  45. Re-visiting the trans insertion model for complexin clamping. Krishnakumar SS, Li F, Coleman J, Schauder CM, Kümmel D, Pincet F, Rothman JE, Reinisch KM. Elife 4 e04463 (2015)
  46. Synip arrests soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-dependent membrane fusion as a selective target membrane SNARE-binding inhibitor. Yu H, Rathore SS, Shen J. J Biol Chem 288 18885-18893 (2013)
  47. Complexin Suppresses Spontaneous Exocytosis by Capturing the Membrane-Proximal Regions of VAMP2 and SNAP25. Malsam J, Bärfuss S, Trimbuch T, Zarebidaki F, Sonnen AF, Wild K, Scheutzow A, Rohland L, Mayer MP, Sinning I, Briggs JAG, Rosenmund C, Söllner TH. Cell Rep 32 107926 (2020)
  48. Formation of Giant Unilamellar Proteo-Liposomes by Osmotic Shock. Motta I, Gohlke A, Adrien V, Li F, Gardavot H, Rothman JE, Pincet F. Langmuir 31 7091-7099 (2015)
  49. Tensor-based morphometry and stereology reveal brain pathology in the complexin1 knockout mouse. Kielar C, Sawiak SJ, Navarro Negredo P, Tse DH, Morton AJ. PLoS One 7 e32636 (2012)
  50. Transmitter release is evoked with low probability predominately by calcium flux through single channel openings at the frog neuromuscular junction. Luo F, Dittrich M, Cho S, Stiles JR, Meriney SD. J Neurophysiol 113 2480-2489 (2015)
  51. Evolutionary Divergence of the C-terminal Domain of Complexin Accounts for Functional Disparities between Vertebrate and Invertebrate Complexins. Wragg RT, Parisotto DA, Li Z, Terakawa MS, Snead D, Basu I, Weinstein H, Eliezer D, Dittman JS. Front Mol Neurosci 10 146 (2017)
  52. Munc18a does not alter fusion rates mediated by neuronal SNAREs, synaptotagmin, and complexin. Zhang Y, Diao J, Colbert KN, Lai Y, Pfuetzner RA, Padolina MS, Vivona S, Ressl S, Cipriano DJ, Choi UB, Shah N, Weis WI, Brunger AT. J Biol Chem 290 10518-10534 (2015)
  53. Synergistic roles of Synaptotagmin-1 and complexin in calcium-regulated neuronal exocytosis. Ramakrishnan S, Bera M, Coleman J, Rothman JE, Krishnakumar SS. Elife 9 e54506 (2020)
  54. Genome-wide association study revealed genomic regions related to white/red earlobe color trait in the Rhode Island Red chickens. Nie C, Zhang Z, Zheng J, Sun H, Ning Z, Xu G, Yang N, Qu L. BMC Genet 17 115 (2016)
  55. Interaction of the Complexin Accessory Helix with Synaptobrevin Regulates Spontaneous Fusion. Vasin A, Volfson D, Littleton JT, Bykhovskaia M. Biophys J 111 1954-1964 (2016)
  56. Complexin splits the membrane-proximal region of a single SNAREpin. Yin L, Kim J, Shin YK. Biochem J 473 2219-2224 (2016)
  57. Reconstituting SNARE-mediated membrane fusion at the single liposome level. Kiessling V, Liang B, Tamm LK. Methods Cell Biol 128 339-363 (2015)
  58. Focused clamping of a single neuronal SNARE complex by complexin under high mechanical tension. Shon MJ, Kim H, Yoon TY. Nat Commun 9 3639 (2018)
  59. Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity. Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, Takao K, Fukaya M, Miyakawa T, Watanabe M, Sakimura K, Manabe T, Igarashi M. J Biol Chem 288 34906-34919 (2013)
  60. A mechanism for exocytotic arrest by the Complexin C-terminus. Makke M, Mantero Martinez M, Gaya S, Schwarz Y, Frisch W, Silva-Bermudez L, Jung M, Mohrmann R, Dhara M, Bruns D. Elife 7 e38981 (2018)
  61. All-atom molecular dynamics simulations of Synaptotagmin-SNARE-complexin complexes bridging a vesicle and a flat lipid bilayer. Rizo J, Sari L, Qi Y, Im W, Lin MM. Elife 11 e76356 (2022)
  62. On the validation of crystallographic symmetry and the quality of structures. Wang J. Protein Sci 24 621-632 (2015)
  63. A BLOC-1-AP-3 super-complex sorts a cis-SNARE complex into endosome-derived tubular transport carriers. Bowman SL, Le L, Zhu Y, Harper DC, Sitaram A, Theos AC, Sviderskaya EV, Bennett DC, Raposo-Benedetti G, Owen DJ, Dennis MK, Marks MS. J Cell Biol 220 e202005173 (2021)
  64. The Accessory Helix of Complexin Stabilizes a Partially Unzippered State of the SNARE Complex and Mediates the Complexin Clamping Function In Vivo. Brady J, Vasin A, Bykhovskaia M. eNeuro 8 ENEURO.0526-20.2021 (2021)
  65. Comment Complexin arrests a neighbor. Weninger KR. Nat Struct Mol Biol 18 861-863 (2011)
  66. Stochastic Properties of Spontaneous Synaptic Transmission at Individual Active Zones. Astacio H, Vasin A, Bykhovskaia M. J Neurosci 42 1001-1019 (2022)
  67. Molecular determinants of complexin clamping and activation function. Bera M, Ramakrishnan S, Coleman J, Krishnakumar SS, Rothman JE. Elife 11 e71938 (2022)
  68. Structure and Function of the Mammalian Neuromuscular Junction. Davis LA, Fogarty MJ, Brown A, Sieck GC. Compr Physiol 12 3731-3766 (2022)
  69. Transfer RNA-Derived Fragments and isomiRs Are Novel Components of Chronic TBI-Induced Neuropathology. Puhakka N, Das Gupta S, Vuokila N, Pitkänen A. Biomedicines 10 136 (2022)
  70. A Role for the V0 Sector of the V-ATPase in Neuroexocytosis: Exogenous V0d Blocks Complexin and SNARE Interactions with V0c. Lévêque C, Maulet Y, Wang Q, Rame M, Rodriguez L, Mochida S, Sangiardi M, Youssouf F, Iborra C, Seagar M, Vitale N, El Far O. Cells 12 750 (2023)
  71. Membrane Binding Induces Distinct Structural Signatures in the Mouse Complexin-1C-Terminal Domain. Grasso EM, Terakawa MS, Lai AL, Xue Xie Y, Ramlall TF, Freed JH, Eliezer D. J Mol Biol 435 167710 (2023)
  72. Complexin-1 regulated assembly of single neuronal SNARE complex revealed by single-molecule optical tweezers. Hao T, Feng N, Gong F, Yu Y, Liu J, Ren YX. Commun Biol 6 155 (2023)
  73. Is it zippered? Does it flare? That darn complexin clamping SNARE. Woodbury DJ. Biophys J 105 835-836 (2013)
  74. Mass spectrometry uncovers intermediates and off-pathway complexes for SNARE complex assembly. Hesselbarth J, Schmidt C. Commun Biol 6 198 (2023)
  75. Roles for diacylglycerol in synaptic vesicle priming and release revealed by complete reconstitution of core protein machinery. Kalyana Sundaram RV, Chatterjee A, Bera M, Grushin K, Panda A, Li F, Coleman J, Lee S, Ramakrishnan S, Ernst AM, Gupta K, Rothman JE, Krishnakumar SS. Proc Natl Acad Sci U S A 120 e2309516120 (2023)
  76. Stability profile of the neuronal SNARE complex reflects its potency to drive fast membrane fusion. Wang S, Ma C. Biophys J 121 3081-3102 (2022)
  77. Synaptophysin chaperones the assembly of 12 SNAREpins under each ready-release vesicle. Bera M, Radhakrishnan A, Coleman J, K Sundaram RV, Ramakrishnan S, Pincet F, Rothman JE. Proc Natl Acad Sci U S A 120 e2311484120 (2023)
  78. Two domains of Tim50 coordinate translocation of proteins across the two mitochondrial membranes. Genge MG, Roy Chowdhury S, Dohnálek V, Yunoki K, Hirashima T, Endo T, Doležal P, Mokranjac D. Life Sci Alliance 6 e202302122 (2023)


Quick links