3rib

X-ray diffraction
2.79Å resolution

Human lysine methyltransferase Smyd2 in complex with AdoHcy

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-lysine methyltransferase SMYD2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 441 amino acids
Theoretical weight: 50.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NRG4 (Residues: 1-433; Coverage: 100%)
Gene names: KMT3C, SMYD2
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 194.56Å b: 57.185Å c: 96.607Å
α: 90° β: 103.95° γ: 90°
R-values:
R R work R free
0.248 0.246 0.3
Expression system: Escherichia coli