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Bacterial oligosaccharyltransferase PglB

X-ray diffraction
3.4Å resolution
PDB 3rce coloured by chain and viewed from the front.
PDB 3rce coloured by chain and viewed from the side.
PDB 3rce coloured by chain and viewed from the top.
Source organism: Campylobacter lari
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero dimer
Accessible surface area: 30300 Å2
Buried surface area: 1700 Å2
Dissociation area: 750 Å2
Dissociation energy (ΔGdiss): 4 kcal/mol
Dissociation entropy (TΔSdiss): 7 kcal/mol
Interface energy (ΔGint): -23 kcal/mol
Symmetry number: 1

Macromolecules

Undecaprenyl-diphosphooligosaccharide--protein glycotransferase
Chain: A
Length: 724 amino acids
Theoretical weight: 84.41 KDa
Source organism: Campylobacter lari
Expression system: Escherichia coli
UniProt:
  • Canonical: B9KDD4 (Residues: 1-712; Coverage: 100%)
Gene names: Cla_1253, pglB
Pfam:
InterPro:
PDB 3rce contains 1 copy of Undecaprenyl-diphosphooligosaccharide--protein glycotransferase in assembly 1. This protein is highlighted and viewed from the front.
PDB 3rce contains 1 copy of Undecaprenyl-diphosphooligosaccharide--protein glycotransferase in assembly 1. This protein is highlighted and viewed from the side.
PDB 3rce contains 1 copy of Undecaprenyl-diphosphooligosaccharide--protein glycotransferase in assembly 1. This protein is highlighted and viewed from the top.
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Substrate Mimic Peptide
Chain: B
Length: 8 amino acids
Theoretical weight: 854 Da
Source organism: Campylobacter lari
Expression system: Not provided
PDB 3rce contains 1 copy of Substrate Mimic Peptide in assembly 1. This protein is highlighted and viewed from the front.
PDB 3rce contains 1 copy of Substrate Mimic Peptide in assembly 1. This protein is highlighted and viewed from the side.
PDB 3rce contains 1 copy of Substrate Mimic Peptide in assembly 1. This protein is highlighted and viewed from the top.
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