X-ray diffraction
3.4Å resolution

Bacterial oligosaccharyltransferase PglB

Source organism: Campylobacter lari
Primary publication:
X-ray structure of a bacterial oligosaccharyltransferase.
Nature 474 350-5 (2011)
PMID: 21677752

Function and Biology Details

Reaction catalysed:
Tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine = tritrans,heptacis-undecaprenyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Undecaprenyl-diphosphooligosaccharide--protein glycotransferase Chain: A
Molecule details ›
Chain: A
Length: 724 amino acids
Theoretical weight: 84.41 KDa
Source organism: Campylobacter lari
Expression system: Escherichia coli
  • Canonical: B9KDD4 (Residues: 1-712; Coverage: 100%)
Gene names: Cla_1253, pglB
Sequence domains:
Substrate Mimic Peptide Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 854 Da
Source organism: Campylobacter lari
Expression system: Not provided

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 85.06Å b: 116.1Å c: 175.04Å
α: 90° β: 90° γ: 90°
R R work R free
0.241 0.238 0.271
Expression systems:
  • Escherichia coli
  • Not provided