Structure analysis

Dpo4 extension ternary complex with 3'-terminal primer t base opposite the 3-methylcytosine (m3c) lesion

X-ray diffraction
2.8Å resolution
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero trimer
Accessible surface area: 20300 Å2
Buried surface area: 6700 Å2
Dissociation area: 1,900 Å2
Dissociation energy (ΔGdiss): 33 kcal/mol
Dissociation entropy (TΔSdiss): 12 kcal/mol
Interface energy (ΔGint): -57 kcal/mol
Symmetry number: 1
Assembly 2
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Multimeric state: hetero trimer

Binding statistics and energies are not available for this assembly

Macromolecules

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Name: DNA (5'-D(*GP*TP*TP*GP*GP*AP*TP*GP*GP*TP*AP*GP*(2DT))-3')
Representative chains: D, H
Source organism: Saccharolobus solfataricus P2 [273057]
Expression system: Not provided
Length: 13 nucleotides
Theoretical weight: 4.06 KDa

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Name: DNA (5'-D(*C*CP*TP*AP*AP*CP*(ME6)P*CP*TP*AP*CP*CP*AP*TP*CP*CP*AP*AP*CP*C)-3')
Representative chains: E, J
Source organism: Saccharolobus solfataricus P2 [273057]
Expression system: Not provided
Length: 20 nucleotides
Theoretical weight: 5.94 KDa

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