› 3rb8

X-ray diffraction
2.6Å resolution

Structure of the phage tubulin PhuZ(SeMet)-GDP

Released:
DOI: 10.2210/pdb3rb8/pdb
PDB entry 3rb8 coloured by chain, front view.
PDB entry 3rb8 coloured by chain, side view.
PDB entry 3rb8 coloured by chain, top view.
Source organism: Pseudomonas phage 201phi2-1
Primary publication:
A phage tubulin assembles dynamic filaments by an atypical mechanism to center viral DNA within the host cell.
Kraemer JA, Erb ML, Waddling CA, Montabana EA, Zehr EA, Wang H, Nguyen K, Pham DS, Agard DA, Pogliano J
Cell 149 1488-99 (2012)
PMID: 22726436

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109848 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phage tubulin-like protein Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 34.99 KDa
Source organism: Pseudomonas phage 201phi2-1
Expression system: Escherichia coli
UniProt:
  • Canonical: B3FK34 (Residues: 2-315; Coverage: 100%)
Gene name: 201phi2-1p059
Structure domains: Tubulin/FtsZ, GTPase domain

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 46.981Å b: 76.062Å c: 92.758Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.163 0.237
Expression system: Escherichia coli

Quick links

Citations

15 review citations

The bacterial cytoskeleton: more than twisted filaments.
Pilhofer et al. (2013)
14 more

1 mention without citation

A robust all-atom model for LCAT generated by homology modeling.
Segrest et al. (2015)