3rae

X-ray diffraction
2.9Å resolution

Quinolone(Levofloxacin)-DNA cleavage complex of type IV topoisomerase from S. pneumoniae

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
4 distinct DNA molecules
Macromolecules (6 distinct):
DNA topoisomerase 4 subunit A Chains: A, B
Molecule details ›
Chains: A, B
Length: 496 amino acids
Theoretical weight: 56.46 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: P72525 (Residues: 1-488; Coverage: 59%)
Gene names: SP_0855, parC
Sequence domains: DNA gyrase/topoisomerase IV, subunit A
DNA topoisomerase 4 subunit B Chains: C, D
Molecule details ›
Chains: C, D
Length: 268 amino acids
Theoretical weight: 30.42 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59961 (Residues: 404-647; Coverage: 38%)
Gene names: SP_0852, parE
Sequence domains:
Structure domains: Rossmann fold
5'-D(*CP*AP*TP*GP*AP*AP*T)-3' Chain: E
Molecule details ›
Chain: E
Length: 7 nucleotides
Theoretical weight: 2.12 KDa
5'-D(P*AP*GP*TP*CP*AP*TP*TP*CP*AP*TP*G)-3' Chain: F
Molecule details ›
Chain: F
Length: 11 nucleotides
Theoretical weight: 3.35 KDa
5'-D(*CP*GP*TP*GP*CP*AP*T)-3' Chain: G
Molecule details ›
Chain: G
Length: 7 nucleotides
Theoretical weight: 2.11 KDa
5'-D(P*GP*AP*CP*TP*AP*TP*GP*CP*AP*CP*G)-3' Chain: H
Molecule details ›
Chain: H
Length: 11 nucleotides
Theoretical weight: 3.36 KDa

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3121
Unit cell:
a: 157.831Å b: 157.831Å c: 211.147Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.186 0.226
Expression system: Escherichia coli