3r6e

X-ray diffraction
1.18Å resolution

The structure of Thermococcus thioreducens' inorganic pyrophosphatase bound to sulfate

Released:
Source organism: Thermococcus thioreducens
Entry authors: Hughes RC, Meehan EJ, Ng JD

Function and Biology Details

Reaction catalysed:
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inorganic pyrophosphatase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 178 amino acids
Theoretical weight: 21.16 KDa
Source organism: Thermococcus thioreducens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: H0USY5 (Residues: 1-178; Coverage: 100%)
Gene names: A3L14_01550, SAMN05216170_1971, ppa
Sequence domains: Inorganic pyrophosphatase
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P1
Unit cell:
a: 62.776Å b: 67.283Å c: 74.139Å
α: 105.92° β: 106.58° γ: 101.92°
R-values:
R R work R free
0.123 0.123 0.148
Expression system: Escherichia coli BL21(DE3)