PDBe 3r66

X-ray diffraction
2.3Å resolution

Crystal structure of human ISG15 in complex with NS1 N-terminal region from influenza virus B, Northeast Structural Genomics Consortium Target IDs HX6481, HR2873, and OR2

Entry authors: Guan R, Ma LC, Krug RM, Montelione GT, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Non-structural protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 113 amino acids
Theoretical weight: 13.12 KDa
Source organism: Influenza B virus (B/Lee/1940)
Expression system: Escherichia coli
  • Canonical: P03502 (Residues: 1-103; Coverage: 37%)
Gene name: NS
Structure domains: S15/NS1, RNA-binding
Ubiquitin-like protein ISG15 Chains: C, D
Molecule details ›
Chains: C, D
Length: 164 amino acids
Theoretical weight: 18.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P05161 (Residues: 1-157; Coverage: 95%)
Gene names: G1P2, ISG15, UCRP
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P1
Unit cell:
a: 39.009Å b: 56.142Å c: 74.02Å
α: 70.59° β: 84.24° γ: 83.91°
R R work R free
0.216 0.214 0.265
Expression system: Escherichia coli