3r5u

X-ray diffraction
1.92Å resolution

The structure of manganese bound Thermococcus thioreducens Inorganic pyrophosphatase

Released:
Source organism: Thermococcus thioreducens
Entry authors: Hughes RC, Coates L, Meehan EJ, Garcia-Ruiz JM, Ng JD

Function and Biology Details

Reaction catalysed:
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inorganic pyrophosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 178 amino acids
Theoretical weight: 20.88 KDa
Source organism: Thermococcus thioreducens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: H0USY5 (Residues: 1-178; Coverage: 100%)
Gene names: A3L14_01550, SAMN05216170_1971, ppa
Sequence domains: Inorganic pyrophosphatase
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: R32
Unit cell:
a: 114.514Å b: 114.514Å c: 148.438Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.152 0.15 0.196
Expression system: Escherichia coli BL21(DE3)