› 3r5u

X-ray diffraction
1.92Å resolution

The structure of manganese bound Thermococcus thioreducens Inorganic pyrophosphatase

Released:
DOI: 10.2210/pdb3r5u/pdb
PDB 3r5u coloured by chain and viewed from the front.
PDB 3r5u coloured by chain and viewed from the side.
PDB 3r5u coloured by chain and viewed from the top.
Source organism: Thermococcus thioreducens
Entry authors: Hughes RC, Coates L, Meehan EJ, Garcia-Ruiz JM, Ng JD

Function and Biology Details

Reaction catalysed: 
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inorganic pyrophosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 178 amino acids
Theoretical weight: 20.88 KDa
Source organism: Thermococcus thioreducens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: H0USY5 (Residues: 1-178; Coverage: 100%)
Gene names: A3L14_01550, SAMN05216170_1971, ppa
Sequence domains: Inorganic pyrophosphatase
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: R32
Unit cell:
a: 114.514Å b: 114.514Å c: 148.438Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.152 0.15 0.196
Expression system: Escherichia coli BL21(DE3)

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