X-ray diffraction
1.8Å resolution

Crystal structures of YwqE from Bacillus subtilis and CpsB from Streptococcus pneumoniae, unique metal-dependent tyrosine phosphatases


Function and Biology Details

Reaction catalysed:
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Tyrosine-protein phosphatase YwqE Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 30.07 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
  • Canonical: P96717 (Residues: 1-254; Coverage: 100%)
Gene names: BSU36240, ywqE
Sequence domains: Capsular polysaccharide synthesis, CpsB/CapC
Structure domains: Metal-dependent hydrolases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P21
Unit cell:
a: 36.624Å b: 59.641Å c: 57.53Å
α: 90° β: 101.08° γ: 90°
R R work R free
0.218 0.216 0.256
Expression system: Escherichia coli