3qxl

X-ray diffraction
2.24Å resolution

Crystal structure of the CDC25 Domain from Ral-specific Guanine-nucleotide Exchange Factor RalGPS1a

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-specific guanine nucleotide-releasing factor RalGPS1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 271 amino acids
Theoretical weight: 31.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JS13 (Residues: 23-289; Coverage: 48%)
Gene names: KIAA0351, RALGEF2, RALGPS1
Sequence domains: RasGEF domain
Structure domains: Ras guanine-nucleotide exchange factors catalytic domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P21
Unit cell:
a: 36.504Å b: 102.035Å c: 82.141Å
α: 90° β: 96.54° γ: 90°
R-values:
R R work R free
0.199 0.197 0.24
Expression system: Escherichia coli