PDBe 3qx3

X-ray diffraction
2.16Å resolution

Human topoisomerase IIbeta in complex with DNA and etoposide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA topoisomerase 2-beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 803 amino acids
Theoretical weight: 91.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02880 (Residues: 450-1206; Coverage: 47%)
Gene name: TOP2B
Sequence domains:
Structure domains:
DNA (5'-D(P*AP*GP*CP*CP*GP*AP*GP*C)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 8 nucleotides
Theoretical weight: 2.44 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*TP*GP*CP*AP*GP*CP*TP*CP*GP*GP*CP*T)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 12 nucleotides
Theoretical weight: 3.65 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: P21
Unit cell:
a: 80.149Å b: 176.801Å c: 94.067Å
α: 90° β: 111.58° γ: 90°
R-values:
R R work R free
0.17 0.168 0.207
Expression systems:
  • Escherichia coli
  • Not provided