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Crystal structure of FAF1 UBX-p97N-domain complex

X-ray diffraction
2Å resolution
PDB entry 3qwz coloured by chain, front view.
PDB entry 3qwz coloured by chain, side view.
PDB entry 3qwz coloured by chain, top view.
Source organism: Homo sapiens
Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Transitional endoplasmic reticulum ATPase and FAS-associated factor 1
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Multimeric state: hetero dimer
Accessible surface area: 12777.54 Å2
Buried surface area: 1711.0 Å2
Dissociation area: 855.5 Å2
Dissociation energy (ΔGdiss): -3.53 kcal/mol
Dissociation entropy (TΔSdiss): 11.02 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-157177
Assembly 2
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Assembly Name: Transitional endoplasmic reticulum ATPase and FAS-associated factor 1
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Multimeric state: hetero tetramer
Accessible surface area: 23311.66 Å2
Buried surface area: 5664.83 Å2
Dissociation area: 2,832.41 Å2
Dissociation energy (ΔGdiss): 1.86 kcal/mol
Dissociation entropy (TΔSdiss): 35.41 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-157181

Macromolecules

Transitional endoplasmic reticulum ATPase
Chain: A
Length: 211 amino acids
Theoretical weight: 23.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55072 (Residues: 1-208; Coverage: 26%)
Gene names: HEL-220, HEL-S-70, VCP
Pfam:
InterPro:
CATH:
Transitional endoplasmic reticulum ATPase in PDB entry 3qwz, assembly 1, front view.
Transitional endoplasmic reticulum ATPase in PDB entry 3qwz, assembly 1, side view.
Transitional endoplasmic reticulum ATPase in PDB entry 3qwz, assembly 1, top view.
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FAS-associated factor 1
Chain: B
Length: 84 amino acids
Theoretical weight: 9.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UNN5 (Residues: 571-650; Coverage: 12%)
Gene names: CGI-03, FAF1, UBXD12, UBXN3A
Pfam: UBX domain
InterPro:
CATH: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
FAS-associated factor 1 in PDB entry 3qwz, assembly 1, front view.
FAS-associated factor 1 in PDB entry 3qwz, assembly 1, side view.
FAS-associated factor 1 in PDB entry 3qwz, assembly 1, top view.
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