› 3qtk

X-ray diffraction
1.85Å resolution

The crystal structure of chemically synthesized VEGF-A

Released:
DOI: 10.2210/pdb3qtk/pdb
PDB entry 3qtk coloured by chain, front view.
PDB entry 3qtk coloured by chain, side view.
PDB entry 3qtk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Total chemical synthesis of biologically active vascular endothelial growth factor.
Mandal K, Kent SB
Angew Chem Int Ed Engl 50 8029-33 (2011)
PMID: 21744452

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147429 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-VEGF Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 102 amino acids
Theoretical weight: 11.95 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P15692 (Residues: 214-315; Coverage: 26%)
Gene names: VEGF, VEGFA
Sequence domains: VEGF heparin-binding domain
Structure domains: Cystine-knot cytokines

Ligands and Environments

3 bound ligands:
Ligand ACT 8 x ACT
Ligand TFA 6 x TFA
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 230.922Å b: 44.008Å c: 73.111Å
α: 90° β: 99.93° γ: 90°
R-values:
R R work R free
0.182 0.18 0.222
Expression system: Not provided

Quick links

Citations

4 review citations

Through the looking glass--a new world of proteins enabled by chemical synthesis.
Kent et al. (2012)
3 more

23 mentions without citation

Chemical synthesis and X-ray structure of a heterochiral {D-protein antagonist plus vascular endothelial growth factor} protein complex by racemic crystallography.
Mandal et al. (2012)
22 more