3qnn

X-ray diffraction
1.92Å resolution

RB69 DNA Polymerase (Y567A) Ternary Complex with dGT Opposite 3tCo

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-directed DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 901 amino acids
Theoretical weight: 104.3 KDa
Source organism: Enterobacteria phage RB69
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q38087 (Residues: 1-901; Coverage: 100%)
Gene name: 43
Sequence domains:
Structure domains:
DNA Template Chain: T
Molecule details ›
Chain: T
Length: 18 nucleotides
Theoretical weight: 5.59 KDa
Source organism: Enterobacteria phage RB69
Expression system: Not provided
DNA Primer Chain: P
Molecule details ›
Chain: P
Length: 13 nucleotides
Theoretical weight: 3.95 KDa
Source organism: Enterobacteria phage RB69
Expression system: Not provided

Ligands and Environments

2 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 74.811Å b: 120.042Å c: 130.409Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.214
Expression systems:
  • Escherichia coli BL21
  • Not provided