› 3qi2

X-ray diffraction
2.8Å resolution

A Galpha P-loop mutation prevents transition to the activated state: G42R bound to RGS14 GoLoco

Released:
DOI: 10.2210/pdb3qi2/pdb
PDB entry 3qi2 coloured by chain, front view.
PDB entry 3qi2 coloured by chain, side view.
PDB entry 3qi2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A P-loop mutation in Gα subunits prevents transition to the active state: implications for G-protein signaling in fungal pathogenesis.
Bosch DE, Willard FS, Ramanujam R, Kimple AJ, Willard MD, Naqvi NI, Siderovski DP
PLoS Pathog 8 e1002553 (2012)
PMID: 22383884

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128999 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 37.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P63096 (Residues: 31-354; Coverage: 92%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Structure domains:
Regulator of G-protein signaling 14 Chains: C, D
Molecule details ›
Chains: C, D
Length: 36 amino acids
Theoretical weight: 4.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O43566 (Residues: 497-532; Coverage: 6%)
Gene name: RGS14
Sequence domains: GoLoco motif

Ligands and Environments

3 bound ligands:
Ligand GDP 2 x GDP
Ligand SO4 2 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 70.003Å b: 131.018Å c: 203.307Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.196 0.273
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

2 review citations

Structural features of the G-protein/GPCR interactions.
Moreira IS. (2014)
1 more

2 mentions without citation

A P-loop mutation in Gα subunits prevents transition to the active state: implications for G-protein signaling in fungal pathogenesis.
Bosch et al. (2012)
1 more