3qgc

X-ray diffraction
1.9Å resolution

Crystal structure of FBF-2 R288Y mutant in complex with gld-1 FBEa A7U mutant

Released:
Source organism: Caenorhabditis elegans
Primary publication:
Stacking interactions in PUF-RNA complexes.
RNA 17 718-27 (2011)
PMID: 21372189

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Fem-3 mRNA-binding factor 2 Chain: A
Molecule details ›
Chain: A
Length: 413 amino acids
Theoretical weight: 47.03 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q09312 (Residues: 164-575; Coverage: 65%)
Gene names: F21H12.5, fbf-2
Sequence domains: Pumilio-family RNA binding repeat
Structure domains: Leucine-rich Repeat Variant
5'-R(*UP*GP*UP*GP*CP*CP*UP*UP*A)-3' Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.81 KDa
Source organism: Caenorhabditis elegans
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P61
Unit cell:
a: 96.577Å b: 96.577Å c: 101.389Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.181 0.21
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided