3qeq

X-ray diffraction
2.59Å resolution

The complex between TCR DMF4 and human Class I MHC HLA-A2 with the bound MART-1(27-35) nonameric peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Melanoma antigen recognized by T-cells 1 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 814 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q16655 (Residues: 27-35; Coverage: 8%)
Gene names: MART1, MLANA
T cell receptor alpha constant Chain: D
Molecule details ›
Chain: D
Length: 194 amino acids
Theoretical weight: 21.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01848 (Residues: 1-86; Coverage: 61%)
Gene names: TCRA, TRAC
Structure domains: Immunoglobulins
DMF4 beta chain Chain: E
Molecule details ›
Chain: E
Length: 243 amino acids
Theoretical weight: 27.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 59.651Å b: 73.663Å c: 225.292Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.215 0.269
Expression systems:
  • Escherichia coli
  • Not provided