X-ray diffraction
2.15Å resolution

Structural characterization of the interaction of colicin A, colicin N, and TolB with TolAIII translocon


Function and Biology Details

Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Tol-Pal system protein TolA Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 13.18 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P19934 (Residues: 302-421; Coverage: 29%)
Gene names: JW0729, b0739, cim, excC, lky, tolA
Sequence domains: TolA C-terminal
Structure domains: Fusion Protein Consisting Of Minor Coat Protein, Glycine Rich Linker, Tola, And A His Tag; Chain: A; Domain 2

Ligands and Environments

1 bound ligand:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3121
Unit cell:
a: 70.283Å b: 70.283Å c: 53.82Å
α: 90° β: 90° γ: 120°
R R work R free
0.194 0.192 0.225
Expression system: Escherichia coli BL21(DE3)