X-ray diffraction
2Å resolution

Crystal structure of bifunctional folylpolyglutamate synthase/dihydrofolate synthase with Mn, AMPPNP and L-Glutamate bound

Source organism: Yersinia pestis CO92
Entry authors: Nocek B, Makowska-Grzyska M, Maltseva N, Anderson W, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Dihydrofolate synthase/folylpolyglutamate synthase Chain: A
Molecule details ›
Chain: A
Length: 437 amino acids
Theoretical weight: 47.55 KDa
Source organism: Yersinia pestis CO92
Expression system: Escherichia coli
  • Canonical: Q0WDC2 (Residues: 1-434; Coverage: 100%)
Gene names: YPO2769, dedC
Sequence domains:
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 62.861Å b: 83.237Å c: 126.99Å
α: 90° β: 90° γ: 90°
R R work R free
0.182 0.171 0.199
Expression system: Escherichia coli