3q8s

X-ray diffraction
2.09Å resolution

Human DNA polymerase iota incorporating dTTP opposite 8-oxo-guanine

Released:
Entry authors: Kirouac KN, Ling H

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase iota Chain: B
Molecule details ›
Chain: B
Length: 420 amino acids
Theoretical weight: 47.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UNA4 (Residues: 26-445; Coverage: 57%)
Gene names: POLI, RAD30B
Sequence domains: impB/mucB/samB family
Structure domains:
DNA (5'-D(*TP*CP*AP*(8OG)P*GP*GP*GP*TP*CP*CP*T)-3') Chain: T
Molecule details ›
Chain: T
Length: 11 nucleotides
Theoretical weight: 3.38 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*AP*GP*GP*AP*CP*CP*C)-3') Chain: P
Molecule details ›
Chain: P
Length: 7 nucleotides
Theoretical weight: 2.11 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P6522
Unit cell:
a: 97.494Å b: 97.494Å c: 201.899Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.226 0.266
Expression systems:
  • Escherichia coli
  • Not provided