3q5u

X-ray diffraction
2.5Å resolution

A minimal NLS from human scramblase 4 complexed with importin alpha

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-156536 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 460 amino acids
Theoretical weight: 49.89 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52293 (Residues: 70-529; Coverage: 87%)
Gene names: Kpna2, Rch1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Phospholipid scramblase 4 Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.49 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9NRQ2 (Residues: 271-283; Coverage: 4%)
Gene names: GIG43, PLSCR4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION ENHANCE ULTRA
Spacegroup: P212121
Unit cell:
a: 77.451Å b: 90.973Å c: 96.895Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.226
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided