PDBe 3pvt

X-ray diffraction
2.03Å resolution

The Phenylacetyl-CoA monooxygenase PaaAC subcomplex with 3-hydroxybutanoyl-CoA

Released:

Function and Biology Details

Reaction catalysed:
Phenylacetyl-CoA + NADPH + O(2) = 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + NADP(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
monomeric
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
1,2-phenylacetyl-CoA epoxidase, subunit A Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 35.79 KDa
Source organism: Escherichia coli str. K-12 substr. MG1655
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P76077 (Residues: 2-309; Coverage: 100%)
Gene names: JW1383, b1388, paaA, ydbO
Structure domains: Ferritin
1,2-phenylacetyl-CoA epoxidase, subunit C Chains: B, C
Molecule details ›
Chains: B, C
Length: 259 amino acids
Theoretical weight: 29.11 KDa
Source organism: Escherichia coli str. K-12 substr. MG1655
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P76079 (Residues: 2-248; Coverage: 100%)
Gene names: JW1385, b1390, paaC, ydbP
Structure domains: Ferritin

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P41212
Unit cell:
a: 77.366Å b: 77.366Å c: 301.412Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.23
Expression system: Escherichia coli BL21(DE3)