PDBe 3pse

X-ray diffraction
2.3Å resolution

Structure of a viral OTU domain protease bound to interferon-stimulated gene 15 (ISG15)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Putative RNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 171 amino acids
Theoretical weight: 19.48 KDa
Source organism: Crimean-Congo hemorrhagic fever orthonairovirus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6TQF5 (Residues: 1-169; Coverage: 4%)
Sequence domains: OTU-like cysteine protease
Structure domains: Cathepsin B; Chain A
Ubiquitin-like protein ISG15 Chain: B
Molecule details ›
Chain: B
Length: 156 amino acids
Theoretical weight: 17.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05161 (Residues: 1-156; Coverage: 95%)
Gene names: G1P2, ISG15, UCRP
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 34.74Å b: 69.81Å c: 69.67Å
α: 90° β: 93.62° γ: 90°
R-values:
R R work R free
0.167 0.164 0.224
Expression system: Escherichia coli