› 3pks

X-ray diffraction
1.75Å resolution

Urate oxidase under 1.5 MPa / 15 bars pressure of nitrous oxide

Released:
DOI: 10.2210/pdb3pks/pdb
PDB entry 3pks coloured by chain, front view.
PDB entry 3pks coloured by chain, side view.
PDB entry 3pks coloured by chain, top view.
Source organism: Aspergillus flavus
Primary publication:
Pressure-response analysis of anesthetic gases xenon and nitrous oxide on urate oxidase: a crystallographic study.
Marassio G, Prangé T, David HN, Santos JS, Gabison L, Delcroix N, Abraini JH, Colloc'h N
FASEB J 25 2266-75 (2011)
PMID: 21421845

Function and Biology Details

Reaction catalysed: 
Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uricase Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.18 KDa
Source organism: Aspergillus flavus
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q00511 (Residues: 2-302; Coverage: 100%)
Gene names: Uox, uaZ
Sequence domains: Uricase
Structure domains: Urate Oxidase;

Ligands and Environments

3 bound ligands:
Ligand AZA 1 x AZA
Ligand NA 1 x NA
Ligand N2O 1 x N2O
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: I222
Unit cell:
a: 80.219Å b: 96.255Å c: 105.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.194
Expression system: Saccharomyces cerevisiae

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Citations

2 review citations

High-pressure macromolecular crystallography and NMR: status, achievements and prospects.
Fourme et al. (2012)
1 more