› 3pjr

X-ray diffraction
3.3Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116698 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
ATP-dependent DNA helicase PcrA Chain: A
Molecule details ›
Chain: A
Length: 724 amino acids
Theoretical weight: 82.6 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P56255 (Residues: 1-724; Coverage: 100%)
Gene name: pcrA
Sequence domains:
Structure domains:
5'-D(*GP*CP*AP*GP*TP*GP*CP*TP*CP*GP*TP*TP*TP*TP*T)-3' Chain: Y
Molecule details ›
Chain: Y
Length: 15 nucleotides
Theoretical weight: 4.58 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
5'-D(*CP*GP*AP*GP*CP*AP*CP*TP*GP*C)-3' Chain: Z
Molecule details ›
Chain: Z
Length: 10 nucleotides
Theoretical weight: 3.03 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P6122
Unit cell:
a: 105.12Å b: 105.12Å c: 380.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.236 0.315
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

105 review citations

Structure and mechanism of helicases and nucleic acid translocases.
Singleton et al. (2007)
104 more

38 mentions without citation

Developing master keys to brain pathology, cancer and aging from the structural biology of proteins controlling reactive oxygen species and DNA repair.
Perry et al. (2007)
37 more