› 3pj5

X-ray diffraction
1.6Å resolution

Crystal structure of far-red fluorescent protein Katushka crystallized at pH 5.0

Released:
DOI: 10.2210/pdb3pj5/pdb
PDB entry 3pj5 coloured by chain, front view.
PDB entry 3pj5 coloured by chain, side view.
PDB entry 3pj5 coloured by chain, top view.
Source organism: Entacmaea quadricolor
Primary publication:
Crystallographic study of red fluorescent protein eqFP578 and its far-red variant Katushka reveals opposite pH-induced isomerization of chromophore.
Pletneva NV, Pletnev VZ, Shemiakina II, Chudakov DM, Artemyev I, Wlodawer A, Dauter Z, Pletnev S
Protein Sci 20 1265-74 (2011)
PMID: 21563226

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-124778 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Red fluorescent protein eqFP578 Chains: A, B
Molecule details ›
Chains: A, B
Length: 224 amino acids
Theoretical weight: 25.51 KDa
Source organism: Entacmaea quadricolor
Expression system: Escherichia coli
UniProt:
  • Canonical: H3JQU5 (Residues: 1-226; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
1 modified residue:
Ligand NRQ 2 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6122
Unit cell:
a: 104.364Å b: 104.364Å c: 217.984Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.187 0.226
Expression system: Escherichia coli

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Citations

14 citation in other articles

Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics.
Akerboom et al. (2013)
13 more

3 mentions without citation

Crystallographic study of red fluorescent protein eqFP578 and its far-red variant Katushka reveals opposite pH-induced isomerization of chromophore.
Pletneva et al. (2011)
2 more