X-ray diffraction
1.95Å resolution

Structure of human O-GlcNAc transferase and its complex with a peptide substrate


Function and Biology Details

Reaction catalysed:
UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit Chains: A, C
Molecule details ›
Chains: A, C
Length: 723 amino acids
Theoretical weight: 80.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: O15294 (Residues: 323-1041; Coverage: 69%)
Gene name: OGT
Sequence domains:
Structure domains:
Casein kinase II subunit alpha Chains: B, D
Molecule details ›
Chains: B, D
Length: 14 amino acids
Theoretical weight: 1.4 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P68400 (Residues: 340-352; Coverage: 3%)
Gene names: CK2A1, CSNK2A1

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Unit cell:
a: 98.6Å b: 136.7Å c: 153.5Å
α: 90° β: 102.9° γ: 90°
R R work R free
0.225 0.224 0.252
Expression systems:
  • Escherichia coli
  • Not provided