3pbs

X-ray diffraction
2Å resolution

Crystal structure of PBP3 complexed with aztreonam

Released:
Source organism: Pseudomonas aeruginosa PAO1
Entry author: Han S

Function and Biology Details

Reaction catalysed:
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidoglycan D,D-transpeptidase FtsI Chain: A
Molecule details ›
Chain: A
Length: 538 amino acids
Theoretical weight: 58.33 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51504 (Residues: 50-579; Coverage: 92%)
Gene names: ALP65_00912, CAZ10_21230, CGU42_01090, E4V10_06485, E5Z62_07090, E5Z63_09665, ECC04_026610, GNQ48_11285, GUL26_13375, IPC111_21940, IPC112_19015, IPC116_09585, IPC117_07395, IPC118_04920, IPC119_07395, IPC120_15550, IPC121_09095, IPC122_04780, IPC124_14230, IPC125_21870, IPC126_24180, IPC127_12935, IPC128_22805, IPC1295_15535, IPC129_24365, IPC1306_24915, IPC1308_23220, IPC1309_06985, IPC130_24445, IPC1311_16270, IPC1312_15480, IPC1313_17325, IPC1315_21965, IPC1316_24135, IPC1317_23495, IPC1318_09915, IPC1319_09685, IPC1320_11910, IPC1321_25630, IPC1322_16515, IPC1323_21620, IPC1324_17780, IPC1328_23340, IPC1329_22455, IPC1330_20130, IPC1331_17140, IPC1333_25770, IPC1334_18700, IPC1336_22140, IPC1337_16335, IPC1339_21875, IPC133_15090, IPC1340_16145, IPC1342_14215, IPC1343_11535, IPC1345_18495, IPC1346_17840, IPC1348_23430, IPC1349_28020, IPC135_19275, IPC137_16810, IPC139_13895, IPC140_17455, IPC144_23880, IPC145_17255, IPC146_16795, IPC1474_21360, IPC1476_21625, IPC1477_15000, IPC1478_19345, IPC1479_03825, IPC147_20975, IPC1480_19760, IPC1481_11065, IPC1482_17070, IPC1485_22740, IPC1486_17305, IPC148_23485, IPC1491_10310, IPC1492_18840, IPC1494_20745, IPC1495_16885, IPC1496_18835, IPC1498_22375, IPC1499_03665, IPC149_19690, IPC1500_25120, IPC1501_27240, IPC1502_22370, IPC1503_25385, IPC1504_21360, IPC1505_04040, IPC1506_20465, IPC1507_10470, IPC1508_21520, IPC1509_19770, IPC150_20385, IPC1510_17840, IPC1511_21000, IPC1512_13610, IPC1513_16690, IPC1514_20090, IPC1515_10850, IPC1516_13270, IPC1517_24555, IPC1518_01875, IPC1519_14090, IPC151_19655, IPC1520_22790, IPC1522_10310, IPC1523_17450, IPC152_11895, IPC1584_03775, IPC1585_15345, IPC1587_21565, IPC1589_28245, IPC1591_18430, IPC1596_21245, IPC1597_23430, IPC1598_08735, IPC1600_19810, IPC1601_18255, IPC1602_14680, IPC1603_16790, IPC1605_28380, IPC1606_28410, IPC161_22390, IPC162_22765, IPC163_25155, IPC164_25610, IPC165_24935, IPC166_24030, IPC167_24555, IPC169_24955, IPC170_23205, IPC171_23005, IPC172_17205, IPC173_11420, IPC174_18480, IPC176_25775, IPC177_15180, IPC178_25080, IPC179_16825, IPC180_12965, IPC181_21340, IPC182_21690, IPC183_11825, IPC27_13015, IPC29_19575, IPC31_15495, IPC32_12355, IPC37_20205, IPC38_24330, IPC40_17705, IPC43_17085, IPC44_18585, IPC45_16465, IPC48_18610, IPC54_25285, IPC55_22610, IPC56_09545, IPC574_19200, IPC575_21310, IPC576_20080, IPC577_15555, IPC578_27125, IPC579_26205, IPC57_10695, IPC580_18590, IPC582_20545, IPC584_24515, IPC586_22885, IPC589_22365, IPC58_16175, IPC596_25775, IPC597_20615, IPC598_25385, IPC599_19915, IPC59_14695, IPC600_21820, IPC601_19795, IPC602_17520, IPC603_15045, IPC604_26500, IPC605_16140, IPC606_14030, IPC607_25595, IPC608_14115, IPC609_21275, IPC60_13915, IPC610_06665, IPC611_20380, IPC612_23580, IPC613_12215, IPC614_11190, IPC615_18845, IPC616_20660, IPC618_04580, IPC61_22670, IPC620_22215, IPC621_20220, IPC622_19205, IPC623_10250, IPC624_27490, IPC625_24025, IPC627_20940, IPC629_18305, IPC630_16550, IPC632_20610, IPC633_21150, IPC634_22690, IPC64_21390, IPC67_11930, IPC68_07130, IPC72_15035, IPC737_20650, IPC73_21415, IPC74_23890, IPC75_23210, IPC76_18105, IPC77_02350, IPC78_21830, NCTC13621_04221, PA52Ts2_1506, ftsI, ftsI1, ftsI_2, pbpB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 67.5Å b: 83.83Å c: 89.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.257
Expression system: Escherichia coli