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Rat Insulin Degrading Enzyme (Insulysin) E111F mutant with two bound peptides

X-ray diffraction
2.142Å resolution
PDB entry 3p7o coloured by chain, front view.
PDB entry 3p7o coloured by chain, side view.
PDB entry 3p7o coloured by chain, top view.
Source organism: Rattus norvegicus
Assemblies composition:
hetero hexamer
hetero trimer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1
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Assembly Name: Insulin-degrading enzyme and peptide
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Multimeric state: hetero hexamer
Accessible surface area: 73898.82 Å2
Buried surface area: 6281.08 Å2
Dissociation area: 2,144.49 Å2
Dissociation energy (ΔGdiss): -7.48 kcal/mol
Dissociation entropy (TΔSdiss): 27.37 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-152948
Assembly 2 (preferred)
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Assembly Name: Insulin-degrading enzyme and peptide
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Multimeric state: hetero trimer
Accessible surface area: 38390.95 Å2
Buried surface area: 1699.77 Å2
Dissociation area: 351.95 Å2
Dissociation energy (ΔGdiss): -0.34 kcal/mol
Dissociation entropy (TΔSdiss): 5.62 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-152945

Macromolecules

Insulin-degrading enzyme
Chain: A
Length: 1019 amino acids
Theoretical weight: 117.88 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P35559 (Residues: 1-1019; Coverage: 100%)
Gene name: Ide
Pfam:
InterPro:
CATH: Metalloenzyme, LuxS/M16 peptidase-like
Insulin-degrading enzyme in PDB entry 3p7o, assembly 2, front view.
Insulin-degrading enzyme in PDB entry 3p7o, assembly 2, side view.
Insulin-degrading enzyme in PDB entry 3p7o, assembly 2, top view.
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active site bound peptide
Chain: B
Length: 8 amino acids
Theoretical weight: 699 Da
Source organism: Rattus norvegicus
Expression system: Escherichia coli
active site bound peptide in PDB entry 3p7o, assembly 2, front view.
active site bound peptide in PDB entry 3p7o, assembly 2, side view.
active site bound peptide in PDB entry 3p7o, assembly 2, top view.
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distal site bound peptide
Chain: C
Length: 7 amino acids
Theoretical weight: 614 Da
Source organism: Rattus norvegicus
Expression system: Escherichia coli
distal site bound peptide in PDB entry 3p7o, assembly 2, front view.
distal site bound peptide in PDB entry 3p7o, assembly 2, side view.
distal site bound peptide in PDB entry 3p7o, assembly 2, top view.
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