› 3p5x

X-ray diffraction
2.2Å resolution

Actinidin from Actinidia arguta planch (Sarusashi)

Released:
DOI: 10.2210/pdb3p5x/pdb
PDB entry 3p5x coloured by chain, front view.
PDB entry 3p5x coloured by chain, side view.
PDB entry 3p5x coloured by chain, top view.
Source organism: Actinidia arguta
Primary publication:
Structural analysis of actinidin and a comparison of cadmium and sulfur anomalous signals from actinidin crystals measured using in-house copper- and chromium-anode X-ray sources.
Yogavel M, Nithya N, Suzuki A, Sugiyama Y, Yamane T, Velmurugan D, Sharma A
Acta Crystallogr D Biol Crystallogr 66 1323-33 (2010)
PMID: 21123873

Function and Biology Details

Reaction catalysed: 
Similar to that of papain.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107622 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actinidin Act1b Chain: A
Molecule details ›
Chain: A
Length: 220 amino acids
Theoretical weight: 23.99 KDa
Source organism: Actinidia arguta
UniProt:
  • Canonical: A5HII2 (Residues: 127-346; Coverage: 62%)
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases

Ligands and Environments

1 bound ligand:
Ligand CD 7 x CD
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E DW
Spacegroup: P212121
Unit cell:
a: 48.519Å b: 56.039Å c: 70.916Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.168 0.222

Quick links

Citations

3 citation in other articles

Utility of anion and cation combinations for phasing of protein structures.
Sharma et al. (2012)
2 more

1 mention without citation

cDNA cloning and molecular modeling of procerain B, a novel cysteine endopeptidase isolated from Calotropis procera.
Singh et al. (2013)