PDBe 3p57

X-ray diffraction
2.19Å resolution

Crystal structure of the p300 TAZ2 domain bound to MEF2 on DNA

Released:
Source organism: Homo sapiens
Primary publication:
Structure of p300 bound to MEF2 on DNA reveals a mechanism of enhanceosome assembly.
OpenAccess logo Nucleic Acids Res. 39 4464-74 (2011)
PMID: 21278418

Function and Biology Details

Reaction catalysed:
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 13-mer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Myocyte-specific enhancer factor 2A Chains: A, B, C, D, I, J
Molecule details ›
Chains: A, B, C, D, I, J
Length: 90 amino acids
Theoretical weight: 10.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02078 (Residues: 2-91; Coverage: 18%)
Gene names: MEF2, MEF2A
Sequence domains: SRF-type transcription factor (DNA-binding and dimerisation domain)
Structure domains: Transcription factor, MADS-box
Histone acetyltransferase p300 Chain: P
Molecule details ›
Chain: P
Length: 112 amino acids
Theoretical weight: 12.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q09472 (Residues: 1726-1835; Coverage: 5%)
Gene names: EP300, P300
Sequence domains: TAZ zinc finger
Structure domains: TAZ domain
DNA (5'-D(*A*AP*AP*CP*TP*AP*TP*TP*TP*AP*TP*AP*AP*GP*A)-3') Chains: E, G, K
Molecule details ›
Chains: E, G, K
Length: 15 nucleotides
Theoretical weight: 4.6 KDa
DNA (5'-D(*TP*TP*CP*TP*TP*AP*TP*AP*AP*AP*TP*AP*GP*TP*T)-3') Chains: F, H, L
Molecule details ›
Chains: F, H, L
Length: 15 nucleotides
Theoretical weight: 4.57 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 74.846Å b: 90.926Å c: 144.802Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.219 0.272
Expression system: Escherichia coli BL21(DE3)