3p43

X-ray diffraction
2.1Å resolution

Structure and Activities of Archaeal Members of the LigD 3' Phosphoesterase DNA Repair Enzyme Superfamily

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LigD_N domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 152 amino acids
Theoretical weight: 17.58 KDa
Source organism: Methanosarcina barkeri str. Fusaro
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q46AP6 (Residues: 1-151; Coverage: 100%)
Gene name: Mbar_A2115
Sequence domains: DNA polymerase Ligase (LigD)

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 30.368Å b: 60.166Å c: 76.471Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.203 0.257
Expression system: Escherichia coli BL21(DE3)