X-ray diffraction
2.6Å resolution

Factor inhibiting HIF-1 Alpha in complex with Notch 1 fragment mouse notch (1997-2016) peptide


Function and Biology Details

Reactions catalysed:
[Ankyrin-repeat domain protein]-L-asparagine + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-asparagine + succinate + CO(2) 
Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O(2) = hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hypoxia-inducible factor 1-alpha inhibitor Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 40.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9NWT6 (Residues: 1-349; Coverage: 100%)
Gene names: FIH1, HIF1AN
Sequence domains: Cupin-like domain
Structure domains:
Notch 1 intracellular domain Chain: B
Molecule details ›
Chain: B
Length: 18 amino acids
Theoretical weight: 1.93 KDa
Source organism: Mus musculus
Expression system: Not provided
  • Canonical: Q01705 (Residues: 1999-2016; Coverage: 1%)
Gene names: Motch, Notch1

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P41212
Unit cell:
a: 86.336Å b: 86.336Å c: 146.45Å
α: 90° β: 90° γ: 90°
R R work R free
0.208 0.208 0.259
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided