X-ray diffraction
2.22Å resolution

Crystal structure of peptidyl-tRNA hydrolase from Mycobacterium smegmatis at 2.2 A resolution

Entry authors: Kumar A, Singh A, Yadav R, Sinha M, Arora A, Sharma S, Singh TP

Function and Biology Details

Reaction catalysed:
N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Peptidyl-tRNA hydrolase Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 20.27 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
  • Canonical: A0R3D3 (Residues: 1-191; Coverage: 100%)
Gene names: MSMEG_5432, MSMEI_5283, pth
Sequence domains: Peptidyl-tRNA hydrolase
Structure domains: Peptidyl-tRNA hydrolase

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 44.909Å b: 58.943Å c: 61.854Å
α: 90° β: 90° γ: 90°
R R work R free
0.193 0.192 0.218
Expression system: Escherichia coli