› 3oyr

X-ray diffraction
2Å resolution

Crystal structure of polyprenyl synthase from Caulobacter crescentus CB15 complexed with calcium and isoprenyl diphosphate

Released:
DOI: 10.2210/pdb3oyr/pdb
PDB entry 3oyr coloured by chain, front view.
PDB entry 3oyr coloured by chain, side view.
PDB entry 3oyr coloured by chain, top view.
Source organism: Caulobacter vibrioides
Primary publication:
Prediction of function for the polyprenyl transferase subgroup in the isoprenoid synthase superfamily.
Wallrapp FH, Pan JJ, Ramamoorthy G, Almonacid DE, Hillerich BS, Seidel R, Patskovsky Y, Babbitt PC, Almo SC, Jacobson MP, Poulter CD
Proc Natl Acad Sci U S A 110 E1196-202 (2013)
PMID: 23493556

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189488 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyprenyl synthetase family protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 37.12 KDa
Source organism: Caulobacter vibrioides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9A6I1 (Residues: 3-338; Coverage: 99%)
Gene name: CC_2113
Sequence domains: Polyprenyl synthetase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

3 bound ligands:
Ligand POP 1 x POP
Ligand DMA 2 x DMA
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 72.894Å b: 72.438Å c: 125.854Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.247
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Enzyme Function Initiative-Enzyme Similarity Tool (EFI-EST): A web tool for generating protein sequence similarity networks.
Gerlt et al. (2015)
14 more

2 mentions without citation

Prediction of function for the polyprenyl transferase subgroup in the isoprenoid synthase superfamily.
Wallrapp et al. (2013)
1 more