3ovs

X-ray diffraction
2.8Å resolution

How the CCA-adding Enzyme Selects Adenine over Cytosine in Position 76 of tRNA

Released:
Source organism: Archaeoglobus fulgidus

Function and Biology Details

Reaction catalysed:
(1a) a tRNA precursor + CTP = a tRNA with a 3' cytidine end + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CCA-adding enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 441 amino acids
Theoretical weight: 51.91 KDa
Source organism: Archaeoglobus fulgidus
UniProt:
  • Canonical: O28126 (Residues: 1-437; Coverage: 100%)
Gene names: AF_2156, cca
Sequence domains:
Structure domains:
RNA (34-MER) Chains: C, D
Molecule details ›
Chains: C, D
Length: 34 nucleotides
Theoretical weight: 10.84 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Not provided

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21212
Unit cell:
a: 111.307Å b: 218.216Å c: 58.369Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.211 0.268
Expression system: Not provided