› 3oti

X-ray diffraction
1.6Å resolution

Crystal Structure of CalG3, Calicheamicin Glycostyltransferase, TDP and calicheamicin T0 bound form

Released:
DOI: 10.2210/pdb3oti/pdb
PDB entry 3oti coloured by chain, front view.
PDB entry 3oti coloured by chain, side view.
PDB entry 3oti coloured by chain, top view.
Source organism: Micromonospora echinospora
Primary publication:
Complete set of glycosyltransferase structures in the calicheamicin biosynthetic pathway reveals the origin of regiospecificity.
Chang A, Singh S, Helmich KE, Goff RD, Bingman CA, Thorson JS, Phillips GN
Proc Natl Acad Sci U S A 108 17649-54 (2011)
PMID: 21987796

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-185170 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP:flavonoid glycosyltransferase YjiC, YdhE family Chains: A, B
Molecule details ›
Chains: A, B
Length: 398 amino acids
Theoretical weight: 43.01 KDa
Source organism: Micromonospora echinospora
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8KND7 (Residues: 2-376; Coverage: 100%)
Gene name: calG3
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

3 bound ligands:
Ligand TYD 2 x TYD
Ligand C0T 2 x C0T
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 45.567Å b: 135.09Å c: 62.093Å
α: 90° β: 95.75° γ: 90°
R-values:
R R work R free
0.153 0.152 0.181
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Recent structures, evolution and mechanisms of glycosyltransferases.
Breton et al. (2012)
6 more

6 mentions without citation

Glycosyltransferase structural biology and its role in the design of catalysts for glycosylation.
Chang et al. (2011)
5 more