X-ray diffraction
2.95Å resolution

Crystal structure of human tRNAHis guanylyltransferase (Thg1) - dGTP complex


Function and Biology Details

Reaction catalysed:
(1a) p-tRNA(His) + ATP = A-Ppp-tRNA(His) + diphosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Probable tRNA(His) guanylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 31.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9NWX6 (Residues: 30-298; Coverage: 90%)
Gene names: ICF45, THG1L
Sequence domains:
Structure domains: Alpha-Beta Plaits

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P43212
Unit cell:
a: 140.912Å b: 140.912Å c: 81.227Å
α: 90° β: 90° γ: 90°
R R work R free
0.254 0.254 0.301
Expression system: Escherichia coli BL21(DE3)