› 3op4

X-ray diffraction
1.6Å resolution

Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from Vibrio cholerae O1 biovar eltor str. N16961 in complex with NADP+

Released:
DOI: 10.2210/pdb3op4/pdb
PDB entry 3op4 coloured by chain, front view.
PDB entry 3op4 coloured by chain, side view.
PDB entry 3op4 coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Primary publication:
Dissecting the Structural Elements for the Activation of β-Ketoacyl-(Acyl Carrier Protein) Reductase from Vibrio cholerae.
Hou J, Zheng H, Chruszcz M, Zimmerman MD, Shumilin IA, Osinski T, Demas M, Grimshaw S, Minor W
J Bacteriol 198 463-76 (2016)
PMID: 26553852

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163868 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier protein] reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 26.7 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand NAP 2 x NAP
Ligand ACT 2 x ACT
1 modified residue:
Ligand MSE 26 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 71.058Å b: 104.095Å c: 126.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.147 0.175
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Indole Biodegradation in Acinetobacter sp. Strain O153: Genetic and Biochemical Characterization.
Sadauskas et al. (2017)
6 more

1 mention without citation

Dissecting the Structural Elements for the Activation of β-Ketoacyl-(Acyl Carrier Protein) Reductase from Vibrio cholerae.
Hou et al. (2016)