› 3onx

X-ray diffraction
2.9Å resolution

Crystal structure of a domain of a protein involved in formation of actin cytoskeleton

Released:
DOI: 10.2210/pdb3onx/pdb
PDB entry 3onx coloured by chain, front view.
PDB entry 3onx coloured by chain, side view.
PDB entry 3onx coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the formin-interaction domain of the actin nucleation-promoting factor Bud6.
Tu D, Graziano BR, Park E, Zheng W, Li Y, Goode BL, Eck MJ
Proc Natl Acad Sci U S A 109 E3424-33 (2012)
PMID: 23161908

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154418 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bud site selection protein 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 141 amino acids
Theoretical weight: 16.5 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P41697 (Residues: 549-688; Coverage: 18%)
Gene names: AIP3, BUD6, L8543.5, YLR319C
Sequence domains: Actin interacting protein 3
Structure domains: Actin interacting protein 3, C-terminal domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 53.307Å b: 67.424Å c: 58.755Å
α: 90° β: 94.51° γ: 90°
R-values:
R R work R free
0.239 0.235 0.278
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Formins at a glance.
Breitsprecher et al. (2013)
2 more

5 mentions without citation

Ligand-induced activation of a formin-NPF pair leads to collaborative actin nucleation.
Graziano et al. (2013)
4 more