PDBe 3onq

X-ray diffraction
2.1Å resolution

Crystal Structure of Regulator of Polyketide Synthase Expression BAD_0249 from Bifidobacterium adolescentis

Released:
Entry authors: Kim Y, Wu R, Tan K, Morales J, Bearden J, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH_30 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 262 amino acids
Theoretical weight: 28.92 KDa
Source organism: Bifidobacterium adolescentis ATCC 15703
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0ZZZ7 (Residues: 1-259; Coverage: 100%)
Gene name: BAD_0249
Sequence domains: PucR C-terminal helix-turn-helix domain
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 85.452Å b: 93.452Å c: 166.687Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.202
Expression system: Escherichia coli BL21