X-ray diffraction
2.15Å resolution

Structure of full-length peroxisomal multifunctional enzyme type 2 from Drosophila melanogaster


Function and Biology Details

Reactions catalysed:
(R)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
(3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H(2)O 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Peroxisomal multifunctional enzyme type 2 Chain: A
Molecule details ›
Chain: A
Length: 613 amino acids
Theoretical weight: 66.17 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9VXJ0 (Residues: 1-598; Coverage: 100%)
Gene names: CG3415, Mfe2
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 114.48Å b: 114.48Å c: 89.11Å
α: 90° β: 90° γ: 90°
R R work R free
0.237 0.234 0.285
Expression system: Escherichia coli BL21(DE3)